Year: 2013 | Month: November | Volume 6 | Issue 4s
Rapid in vitro Multiplication of Eulophia Cullenii (Wight) Blume - a Rare, Endemic and Exquisite Orchid of Southern Western Ghats
Abstract: <div>As part of a conservation programme to multiply Western Ghats orchids, green pod culture of Eulophia cullenii, a rare, endemic exquisite and therapeutically important orchid of Western Ghats was attempted. Green pod culture of E. cullenii, was tested in three different culture media viz. Mitra et al., Knudson C, and Wimber media. Mature seeds released from a three month old the green pod of E. cullenii were cultured in the above mentioned liquid nutrient media supplemented with different organic additives</div><div>like casein acid hydrolysate (CH), peptone (P), yeast extract (YE) and coconut water (CW). Mitra nutrient medium supplemented with 0.05% CH (w/v) supported highest percentage (70%) seed germination. CW was found be inhibitory for seed germination in all the media tried. Protocorm after 60 days in liquid medium were transferred to solid Mitra medium containing same additives. Protocorm proliferation was noticed in Mitra basal and organic supplemented medium and maximum of an average of 15 daughter protocorms within 8 weeks of culture in solid basal Mitra medium. Proliferated protocorm when transferred to Mitra basal medium within 8 weeks leafy shoots with root initials and initiation of in vitro rhizome were obtained. These seedling again transferred to fresh basal medium healthy seedling with in vitro derived rhizome were formed within 8 weeks of culture. After the third subculture in solid medium, the healthy rooted seedlings with in vitro developed rhizome obtained were transferred to community pots</div><div>containing sand and farmyard manure (1:1) and 79% survival rate was obtained after one year of establishment.</div>
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